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2 edition of molecular study of the viral proteins of infectious hematopoietic necrosis virus found in the catalog.

molecular study of the viral proteins of infectious hematopoietic necrosis virus

Ya-li HsuМ€

molecular study of the viral proteins of infectious hematopoietic necrosis virus

by Ya-li HsuМ€

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  • 11 Currently reading

Published .
Written in English


Edition Notes

Other titlesViral proteins., Infectious hematopoietic necrosis virus.
Statementby Ya-li Hsu.
The Physical Object
Pagination[11], 135 leaves, bound :
Number of Pages135
ID Numbers
Open LibraryOL15080890M

Title: A Molecular Study of the Viral Proteins of Infectious Hematopoietic Necrosis Virus Abstract approved: Redacted for privacy-7 Jo-Ann Ching Leo g The virion protein patterns of 72 isolates of infectious hematopoietic necrosis virus (IHNV) were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of S-methionine. Infectious hematopoietic necrosis virus (IHNV) is a pathogen of major economic importance to the aquaculture industry. The long-term goal of our work is to develop a safe and effective recombinant IHNV vaccine and possibly use IHNV as a virus vector to express foreign : Arun Ammayappan.

Infectious hematopoietic necrosis virus (IHNV) is one of the most important pathogens of salmonid fish. In this study a comprehensive phylogenetic analysis of the genetic evolution and variety of Austrian IHNV strains, as well as selected strains ensuring worldwide coverage, is by: The genome RNA and six mRNA species of infectious haematopoietic necrosis virus were analysed by denaturing gel electrophoresis. The following molecular weights were determined: genome RNA, × ; mRNA 1, × ; mRNA 2, × ; mRNA 3, × ; mRNA 4 (containing two different mRNA species), × ; and mRNA 5, × Cited by:

Molecular biological approaches were used to study and interfere with the life cycle of infectious hematopoietic necrosis virus (IHNV). These included the control of IHN disease in rainbow trout by genetic immunization or interference in vitro by synthesis of sense and antisense expression of the viral nucleocapsid (N) gene, and in situ Author: Eric David Anderson. Plasmids carrying cDNA sequences to the mRNA species of infectious hematopoietic necrosis virus were constructed and cloned into Escherichia coli. Characterization of 21 cloned plasmids by hybridization to mRNA blots identified sets of plasmids with homology to each of the six viral mRNA by:


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Molecular study of the viral proteins of infectious hematopoietic necrosis virus by Ya-li HsuМ€ Download PDF EPUB FB2

Cells and Viruses. The IHNV strain was kindly provided by Scott LaPatra, Clear Springs Foods Inc., Idaho, USA. The virus was initially recovered from acutely infected juvenile rainbow trout during routine examinations of hatchery-reared fish, conducted from Cited by: The virion protein patterns of 72 isolates of infectious hematopoietic necrosis virus (IHNV) were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of S-methionine labeled virus.

This analysis led to the classification of these virus isolates into 4 Author: Ya-li Hsu. Infectious hematopoietic necrosis virus (IHNV) is the type species of the genus Novirhabdovirus, within the family Rhabdoviridae, infecting several species of wild and hatchery reared salmonids.

Similar to other rhabdoviruses, IHNV has a linear single-stranded, negative-sense RNA genome of approximat by: Abstract.

The fish rhabdoviruses, infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV), cause extensive losses among salmon and trout in several areas of the world (Bootland and Leong, ; Smail, ; Wolf, ).Cited by: The role of viral proteins in the pathogenesis of infectious hematopoietic necrosis virus (IHNV) was studied at the molecular level.

The expression of the viral genes at the protein and RNA level, and their cellular localization, were characterized to further our understanding of viral pathogenesis. The pathogenic effect of individual viral proteins was also investigated and a method for detecting viral RNA Author: Pinwen Peter Chiou.

The route of entry and progression of infectious hematopoietic necrosis virus in Oncorhynchus mykiss (Walbaum): a sequential immunohistochemical study. The sendai virus nonstructural C proteins specifically inhibit viral mRNA synthesis. tion about the infectious hematopoietic necrosis virus (IHNV) isolates used in this study for comparison and phylogenetic analysis).

Results The complete nucleotide sequence of The entire genome of IHNV strain was amplified as six overlapping cDNA fragments that were cloned, and the DNA was sequenced (Fig.

The completeCited by: Similarly, the complete genome comparison of MI03GL strain with different members of Rhabdoviridae family shows % identity, but among novirhabdoviruses, it exhibits 56% identity with infectious hematopoietic necrosis virus (IHNV) and 62% with snakehead rhabdovirus (SHRV), as shown in Table Table5.

Also in novirhabdoviruses, it is evident that non-virion protein (which is absent in other Cited by: The virion protein patterns of 71 isolates of infectious hematopoietic necrosis virus (IHNV) from the Pacific Northwest were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Infectious hematopoietic necrosis virus RNA genome consists of approximately 11 kilobases, which encodes for six viral proteins: nucleoprotein (N), polymerase-associated phosphoprotein (P), matrix protein (M), glycoprotein (G), non-structural protein (NV), and RNA-dependent RNA polymerase (L) (Kurath et al., ).

The N protein interacts with the viral RNA genome to form the ribonucleoprotein. Table 1. Estimated number and molecular weight of infectious hematopoietic necrosis virus proteins (polymerase), 67, for G (glycoprotein), 40, for N (nucleocapsid protein), 22, for M1 (matrix protein), for M2 (materix protein) (Fig.

The relative contribution of each virion 1proCited by: The fish rhabdoviruses, infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV), cause extensive losses among salmon and trout in several areas of the world (Bootland and Leong, ; Smail, ; Wolf, ).

Historically, IHNV was endemic among wild anadromous salmonids in the western portion of North America, but the virus has spread to stocks of Cited by: This study determined the complete genomic sequence of the infectious hematopoietic necrosis virus (IHNV) strain Ch isolated from farmed brook trout (Salvelinus fontinalis) that died from a disease caused by the virus in northeast China.

The sequence was determined from 10 overlapping clones obtained through RT-PCR by: Vertical transmission of infectious hematopoietic necrosis virus in sockeye salmon (Oncorhynchus nerka): isolation of virus from dead eggs and fry. Viral protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its anisense RNA.

Infectious hematopoietic necrosis virus (IHNV), the aetiologic agent of infectious haematopoietic necrosis (IHN), is a major viral pathogen of salmonids that causes serious economic losses worldwide.

IHNV causes acute clinical infections and sudden mortalities in fry and juveniles and is transmitted horizontally and vertically. A molecular study of the viral proteins of infectious hematopoietic necrosis virus.

Abstract. Graduation date: The virion protein patterns of 72 isolates of infectious\ud hematopoietic necrosis virus (IHNV) were analyzed by sodium dodecyl\ud sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of S-methionine\ud labeled virus.

Introduction. Infectious hematopoietic necrosis virus (IHNV) is an important viral pathogen which causes a lethal disease in wild and cultured salmonid species (Nichol et al., ; Adel et al., ).Due to its very contagious nature, IHNV may lead to cumulative mortality of % depending on the species and size of fish and environmental : Seyed Amir Hossein Jalali, Rezvan Mohammadinezhad, Ashraf Mohammadi, Mohamad Hassan Latifian, Majid.

Infectious hematopoietic necrosis virus (IHNV), a member of the genus Novirhabdovirus and family Rhabdoviridae, causes infectious hematopoietic necrosis in fish. The virus has the typical bullet shape of members of the Rhabdoviridae, and the genome consists of a single molecule of negative-sense ssRNA with varying strain pathogenicity.

IHNV isolates can be grouped into three genetic types, which are. Infectious hematopoietic necrosis virus (IHNV), one of the most important pathogenic fish viruses, affects trout fisheries and causes considerable economic losses. Currently, in Korea, more. ELSEVIER Virus Research 38 ()Virus Research Molecular epizootiology and evolution of the glycoprotein and non-virion protein genes of infectious hematopoietic necrosis virus, a fish rhabdovirus Stuart T.

Nichol a,*, Joan E. Rowe a, James R. Winton b a Departments of Biochemistry and Microbiology, University of Nevada, Reno, NV, USA b National Biological Service, Northwest Cited by:. Infectious hematopoietic necrosis virus (IHNV) is among the most important pathogens affecting the salmonid industry.

Here, we investigated the molecular evolution and circulation of isolates from. Infectious hematopoietic necrosis virus RNA genome consists of approximately 11 kilobases, which encodes for six viral proteins: nucleoprotein (N), polymerase-associated phosphoprotein (P), matrix protein (M), glycoprotein (G), non-structural protein (NV), and RNA-dependent RNA polymerase (L) (Kurath et al., ).

The N protein interacts with Cited by: 1.Infectious hematopoietic necrosis virus (IHNV) is the type species of the genus Novirhabdovirus, within the family Rhabdoviridae, infecting several species of wild and hatchery reared salmonids.