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Wednesday, July 29, 2020 | History

2 edition of Identification of members ofthe P-glycoprotein metigene family. found in the catalog.

Identification of members ofthe P-glycoprotein metigene family.

William F.* Ng

Identification of members ofthe P-glycoprotein metigene family.

by William F.* Ng

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Published .
Written in English


The Physical Object
Pagination67 leaves
Number of Pages67
ID Numbers
Open LibraryOL14751669M

  Glucose 1-phosphate gained by means of glycogen phosphorylase, is converted into glucose 6-phosphate that will be transported into endoplasmic reticulum where glucose 6-phosphate is divided into inorganic phosphate (Pi) and glucose , glucose is released form E.R. by the help of its transported, as it is true for Pi. There are many methods utilized in glycoprotein detection and identification, with the most common being SDS-PAGE, mass spectroscopy, HPLC, NMR and Western blotting. It is helpful and often necessary to include glycoproteins as standards and/or molecular weight markers. In addition to these specific glycoprotein standards, Sigma offers a broad.

  The in vivo data strongly support the hypothesis that inhibition of P-glycoprotein-mediated digoxin elimination plays an important role in the increase of plasma digoxin concentration occurring with quinidine coadministration in wild-type mice and thus support a . Atazanavir sulfate Modulator % Atazanavir sulfate (BMS sulfate) is a highly selective HIV-1 protease inhibitor for the treatment of HIV infection, and is the first protease inhibitor approved for once-daily administration. Atazanavir sulfate (BMS sulfate) is a substrate and inhibitor of CYP3A4, and an inhibitor and inducer of P-glycoprotein (P-gp).

P-glycoprotein (P-gp) is a kDa membrane protein and the gene product of the multiple drug resistance (MDR1 or ABCB1) constitutes an important part of the blood–brain barrier and actively exports a number of molecules across the blood–brain barrier back into the vascular space, subsequently reducing central nervous system (CNS) bioavailability of these substances. Purchase Glycoprotein and Proteoglycan Techniques, Volume 16 - 1st Edition. Print Book & E-Book. ISBN ,


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Identification of members ofthe P-glycoprotein metigene family by William F.* Ng Download PDF EPUB FB2

This protein appears to be encoded by a multigene family. Thus, differential expression of different members of this family may contribute to the complexity of the multidrug resistance phenotype.

Three lambda genomic clones isolated from a hamster genomic library represent different members of the hamster P-glycoprotein gene by: Jill E Maddison, Stephen W Page, in Small Animal Clinical Pharmacology (Second Edition), P-glycoprotein.

P-glycoprotein (P-gp) or multidrug resistance protein 1 (Mdr1) is a member of the ATP binding cassette (ABC) superfamily of transmembrane transport proteins and in the dog is encoded by the gene Mdr1.P-gp is an important drug efflux transporter that has a significant impact on the.

Ng WF, Sarangi F, Zastawny RL, Veinot DL, Ling V. Identification of members of the P-glycoprotein multigene family. Mol Cell Biol. ; – [PMC free article] Nielsen D, Skovsgaard P-glycoprotein as multidrug tranporter: a critial review of current multidrug restistnat cell lines.

Biochim Biophys Acta. ; –Cited by:   Drug resistant variant cell lines were selected in vitro for resistance to adriamycin or vincristine, P/ADR and P/VCR, respectively, from P murine leukemia cells. These cells were demonstrated by immunoblot studies with P-glycoprotein specific monoclonal antibody (C) to overexpress not only P-glycoprotein but also approximately 65 kDa protein, and the expression Cited by:   Semin Onco; Gerlach JH, Endicott JA, Juranka PF, et al.

Homology between P­glycoprotein and a bacterial hemolysin transport protein suggests a model for multidrug resistance. Nature ; Ng WF, Sarangi F, Zastawny RL, et al.

Identification of members of the P-glycoprotein multigene by: P-glycoprotein is an important mediator of drug-drug interactions. 3 The pharmacokinetics of a drug may be altered when co-administered with compounds which inhibit or induce P-glycoprotein.

3, 5, 6 Inhibitors include clarithromycin, erythromycin, ritonavir and. P-glycoproteins, encoded by families of evolutionarily conserved genes, can confer a multidrug-resistant phenotype to mammalian tumor cells.

To obtain more information on their functions in normal cells we have cloned genomic and complementary DNA sequences of four P-glycoprotein gene homologs of the genetically well-characterized nematode Caenorhabditis elegans, termed pgp-1, pgp. Dey et al. [] identified the molecular evidence and functional expression of P-gp (MDR1) in human and rabbit cornea and in corneal epithelial cell same group demonstrated the role of P-gp as a barrier to ocular erythromycin absorption in rabbits following single-dose infusion studies [].Radiolabeled cyclosporine-A (Cys-A) transport was studied across cultured rabbit corneal.

The initial discovery of p-glycoprotein in the plasma membrane of MDR cancer cell lines was followed quickly by the cloning of its gene. Sequence analysis of cloned cDNAs revealed that p-glycoprotein was a member of the ABC family of membrane transporters.

Subsequent biochemical characterization demonstrated the binding of chemotherapeutic drugs and ATP to p-glycoprotein. P-Glycoprotein inhibitors can and do affect the concentrations of other drugs.

I will be honest in that when I think about drug interactions, it isn’t the first thing that comes to mind. This gut transporter can impact clinical care and influence drug decisions.

This protein is extensively expressed in the intestinal epithelium, hepatocytes, renal proximal tubular cells, and luminal membrane of the endothelial cells of the BBB where it transports compounds out of the brain. P-glycoprotein (abbreviated as P-gp or Pgp) is also called ABCB1 (ATP-ABC subfamily B member 1) or MDR1 (for multiple drug.

Proc. Natl. Acad. Sci. USA89() Table 1. Drug-resistance properties oftransfectants Cell line Drug ED50, ng/ml Foldresistance A 2 2 4 r5 Control DC-3F(parental) (antisense negative control) Transfectant ActD Colc Vinc Daun.

P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp) also known as multidrug resistance protein 1 (MDR1) or ATP-binding cassette sub-family B member 1 (ABCB1) or cluster of differentiation (CD) is an important protein of the cell membrane that pumps many foreign substances out of cells.

More formally, it is an ATP-dependent efflux pump with broad substrate. Aims. The CT polymorphism in the human MDR1 gene is associated with lower intestinal P-glycoprotein expression, reduced protein function in peripheral blood cells and higher plasma concentrations of the P-glycoprotein substrate digoxin.

Using fexofenadine, a known P-glycoprotein substrate, the hypothesis was tested whether this polymorphism also affects the disposition of other.

P-Glycoprotein (Pgp) was isolated from CHRC5 membranes by selective detergent extraction and further purified by lentil lectin affinity chromatography.

The purified product displayed a very high basal ATPase activity ( mumol/min per mg protein in the absence of added drugs or lipids) with an apparent Km for ATP of mM. Human p-glycoprotein-1 ( aa, kDa) is encoded by the human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) gene.

The protein is an ATP-dependent, transmembrane transport/efflux pump glycoprotein that is expressed in liver, kidney, small intestine and brain.

The permeability-glycoprotein (P-gp) is the first ABC-transporter identified in human. It is also termed ABCB1 or MDRA and eliminates a variety of structurally unrelated compounds from the cell.

P-gp. P-glycoprotein has a modest role in drug elimination. It is expressed in the luminal membrane of proximal tubule cells in the kidneys.

P-glycoprotein pumps drugs into the urine. Drug interactions P-glycoprotein is an important mediator of drug-drug interactions.3 The.

P-glycoprotein (Pgp), the product of the human MDR1 gene responsible for a major form of multidrug resistance in tumor cells, is a transmembrane protein that carries out ATP-dependent efflux of various lipophilic compounds, including many anticancer drugs. Published: September Publications Medicines interactions: the role of P-glycoprotein.

Prescriber Update 32(3): September P-glycoprotein is the most well known of the transmembrane efflux transporters and first surfaced in the s as the reason for multidrug resistance in cancer cells. P-glycoprotein, the most extensively studied ATP-binding cassette (ABC) transporter, functions as a biological barrier by extruding toxins and xenobiotics out of cells.

In vitro and in vivo studies have demonstrated that P-glycoprotein plays a significant role in drug absorption and disposition. Because of its localisation, P-glycoprotein appears to have a greater impact on limiting cellular. A kDa transmembrane glycoprotein from the superfamily of ATP-BINDING CASSETTE TRANSPORTERS.

It serves as an ATP-dependent efflux pump for a variety of chemicals, including many ANTINEOPLASTIC.Many drugs commonly used in veterinary patients are capable of inhibiting P‐glycoprotein function and thereby causing an interaction that results in severe chemotherapeutic drug toxicity.

The intent of this review is to describe the mechanism and clinical implications of drug–drug interactions involving P‐glycoprotein and anticancer drugs.